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KMID : 1161520090130010017
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Animal Cells and Systems
2009 Volume.13 No. 1 p.17 ~ p.23
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Annexin I stimulates insulin secretion through regulation of cytoskeleton and PKC activity
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Kang Na-Na
Won Jong-Hak
Park Young-Min
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Abstract
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In previous studies, we found that Annexin I (Anx I) was co?secreted with insulin in response to glucose, and that extracellular Anx I stimulated the release of insulin via the Anx I binding site in rat pancreatic islets and the β?cell line. However, the role that Anx I plays in the insulin secretion was not established. Therefore, in this study, we evaluated the insulin secretion pattern in response to Anx I and the involvement of the cytoskeleton or PKC in Anx I?stimulated insulin secretion in MIN6N8a cells. The peak time of insulin secretion in response to Anx I treatment corresponded with the second phase insulin secretion by glucose in the perifused pseudoislets. In addition, Anx I?stimulated insulin secretion was not affect by readily releasable pool depletion. Taken together, these findings indicate that Anx I treatment was associated with movement of the reserve pool of insulin. Furthermore, Anx I?stimulated insulin secretion was attenuated by treatment with a microfilament inhibitor, cytochalasin B, as well as by PKC down regulation. These results indicate that Anx I may be a regulator of second phase insulin secretion.
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KEYWORD
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Anx I, insulin secretion, cytoskeleton, perifusion, reserve pool
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